Sialyiation of N-linked glycoproteins in the baculovirus expression vector system 

Patent No. 6,472,175

Issued: October 29, 2002

Patent No. 6,261,805

Issued: July 17, 2001

Inventor:  H. Alan Wood, Ithaca, NY

Assignee: Boyce Thompson Institute for Plant Research, Inc., Ithaca, NY
Contact: Christopher Michaels, at Brown & Michaels, Ithaca (see below for address and phone number) -

The present disclosure utilizes a novel approach to protein preparation in the baculovirus expression vector system (BEVS). Specifically, the present invention analyzes the effects of microgravity/low shear on complex glycosylation of proteins prepared via BEVS, including the addition of terminal sialic acid residues to N-linked oligosaccharides. 

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