Enzymatic cleavage based lateral flow assays
Patent No. 9,212,386
Issued: December 15, 2015
Inventors: Babu; Uma Mahesh (Bradenton, FL), Sambursky; Robert P.
(Bradenton, FL), VanDine; Robert W. (Montoursville, PA)
Methods and devices for detecting a target enzyme include an anchored or trapped peptide complex. The peptide complex includes an anchor particle immobilized on a sample analysis device or trapped in a reaction receptacle including a filter, a peptide, with at least one enzyme cleavage site for a target enzyme, bound to the anchor particle, at least one detectable label, and at least one first tag bound to the peptide on a side of the enzyme cleavage site opposite the anchor particle. When the target enzyme is present in the sample, the enzyme cleaves the peptide at the enzyme cleavage site, permitting the cleaved peptide to reach the test zone of a sample analysis device such that the first tag binds to the immobilized second tag and a signal is detected at the test zone.
Reference to Related Applications
This application claims one or more inventions which were disclosed in Provisional Application No. 61/510,681, filed Jul. 22, 2011, entitled "ENZYMATIC CLEAVAGE BASED LATERAL FLOW ASSAYS". The benefit under 35 USC .sctn.119(e) of the United States provisional application is hereby claimed, and the aforementioned application is hereby incorporated herein by reference.
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