Isolating a mammalian embryonic stem cell from a homogenous pluripotent outgrowth of a mammalian pre-implantation embryo
Patent No. 9,499,785
Issued: November 22, 2016
Inventor: Vassiliev; Ivan (Urrbrae, AU), Nottle; Mark Brenton
The present invention provides a method of isolating a pluripotent cell from a pre-implantation embryo without isolation of the pluripotent cells from other cells, the method including propagating a whole pre-implantation embryo including one or more pluripotent cells, embedded in a feeder cell layer and cultivated in a medium substantially free of serum, and isolating a pluripotent cell from the one or more pluripotent cells. The present invention also provides pluripotent cells generated by the method and uses thereof.
Reference to Related Applications
This application claims priority from Australian Provisional Patent Application No. 2006905889 filed on 24 Oct. 2006, the contents of which are to be taken as incorporated herein by this reference.
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