Isolating a mammalian embryonic stem cell from a homogenous pluripotent outgrowth of a mammalian pre-implantation embryo

Patent No. 9,499,785

Issued: November 22, 2016
Filed: October 24, 2007
PCT Filed: October 24, 2007
PCT No.: PCT/AU2007/001619
371(c)(1),(2),(4) Date: April 23, 2009
PCT Pub. No.: WO2008/049161
PCT Pub. Date: May 02, 2008
Term Extension: 66 days

Inventor: Vassiliev; Ivan (Urrbrae, AU), Nottle; Mark Brenton (Bibaringa, AU)
Assignee: ICMSTEMCELL PTY LTD (Bibaringa, South Australia, AU)


The present invention provides a method of isolating a pluripotent cell from a pre-implantation embryo without isolation of the pluripotent cells from other cells, the method including propagating a whole pre-implantation embryo including one or more pluripotent cells, embedded in a feeder cell layer and cultivated in a medium substantially free of serum, and isolating a pluripotent cell from the one or more pluripotent cells. The present invention also provides pluripotent cells generated by the method and uses thereof.

Reference to Related Applications

This application claims priority from Australian Provisional Patent Application No. 2006905889 filed on 24 Oct. 2006, the contents of which are to be taken as incorporated herein by this reference.


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